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Lambda PCR Kit
In this experiment, students will learn the basic concepts of PCR by observing the increased accumulation of product with increasing number of cycles. The experiment is divided into three modules - PCR Reaction, Separation of PCR Reactions by Electrophoresis, and Size Determination of PCR Amplified DNA Fragment. In this experiment the template DNA to be amplified is a linear molecule from the E. coli bacteriophage lambda. It contains approximately 49,000 base pairs and has 5 recognition sites for Eco RI, and 7 for Hind III. This experiment contains reagents for 10 groups to perform the PCR reactions. Kit contents: Features PCR Dryzyme® reaction pellets™ Instastain® Ethidium Bromide DNA stain UltraSpec-Agarose™ Electrophoresis Buffer (50x) 10x Gel Loading Solution InstaStain® Ethidium Bromide Microcentrifuge Tubes (0.5 ml) PCR tubes (0.2 ml) Wax beads (for waterbath option or thermal cyclers without heated lid) Coupon for a freezer pack with the following reagents (ship and store at -20°C): Primer Mix (two primers) 200 base pair ladder Lambda DNA Template for Amplification Requires, but does not include include a horizontal gel electrophoresis apparatus, DC power supply, thermal cycler, balance, microcentrifuge, waterbath, UV transilluminator or UV photodocumentation system, microwave, automatic pipets, distilled or deionized water, and isopropynol.
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