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Lambda PCR Kit
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In this experiment, students will learn the basic concepts of PCR by observing the increased accumulation of product with increasing number of cycles. The experiment is divided into three modules - PCR Reaction, Separation of PCR Reactions by Electrophoresis, and Size Determination of PCR Amplified DNA Fragment. In this experiment the template DNA to be amplified is a linear molecule from the E. coli bacteriophage lambda. It contains approximately 49,000 base pairs and has 5 recognition sites for Eco RI, and 7 for Hind III. This experiment contains reagents for 10 groups to perform the PCR reactions.
Kti contents:
· Features PCR Dryzyme® reaction pellets™
· Instastain® Ethidium Bromide DNA stain
· UltraSpec-Agarose™
· Electrophoresis Buffer (50x)
· 10x Gel Loading Solution
· InstaStain® Ethidium Bromide
· Microcentrifuge Tubes (0.5 ml)
· PCR tubes (0.2 ml)
· Wax beads (for waterbath option or thermal cyclers without heated lid)
· Coupon for a freezer pack with the following reagents (ship and store at -20°C):
· Primer Mix (two primers)
· 200 base pair ladder
· Lambda DNA Template for Amplification
Requires, but does not include include a horizontal gel electrophoresis apparatus, DC power supply, thermal cycler, balance, microcentrifuge, waterbath, UV transilluminator or white light, microwave, automatic pipets, distilled or deionized water, and isopropynol.
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