Students are introduced to the basic concepts of DNA fingerprinting, commonly used for medical and forensic procedures as well as paternity determinations. Students have a chance to crack a hypothetical criminal case. Provided are DNA samples from the scene of the crime, Suspect #1 and Suspect #2. Remember: no two individuals have exactly the same pattern of restriction enzyme sites. Reagents for 6 gels-2 to 4 students per gel. Kit contents (room temperature storage):

• Ultraspec-Agarose
• Electrophoresis Buffer (50X)
• 6 ready-to-load DNA samples (Does not contain human DNA)
• Methylene Blue Plus
• 1mL pipet
• DNA Blue InstaStain
• Complete instructions
• Transfer micropipets

Required but not included: horizontal cell, power supply, standard glassware, beaker tongs, distilled or deionized H₂O. Highly recommended: staining tray package, visible light box, automatic micropipet to deliver 40µl.