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Transformation of E. Coli with pGAL DNA
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(EDVO-Kit 221)
With the newly designed plasmid pGAL transformed E. coli cells will express the
same 2 phenotypic traits as plasmid pUC8: ampicillin resistance (formation of
colonies) and a blue color. However, unlike pUC8, pGAL contains the entire lac Z gene for B-galactosidase
and inducer IPTG is not required. The blue color is the result of the cleavage
of the X-GAL (substrate) by B-galactosidase (enzyme). The lac operon is also
discussed. Reagents for 10 groups, 3- students per group.
Kit contents:
• Coupon for perishable items requiring
refrigerator or freezer storage |
• Luria broth agar |
| • E. coli (freeze-dried LyphoCells™) |
• Luria recovery broth |
| • Supercoiled pGAL DNA |
• Petri plates |
| • Control buffer |
• Transfer micropipets |
| • Ampicillin |
• 10mL pipets |
| • X-Gal in solvent |
• Inoclating loops |
| • Cell reconstitution media |
• Microtest tubes |
Required, but not included: water baths (to set at 37 and 42°C), incubator/oven (37°C), hot plate or microwave oven, automatic micropipet to deliver 25µl, ice.
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Other Items to Consider:
Rod Assembly, 4" x 6"
40-Place Set Tube Support
Torso, Mini
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