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Lab 4: Plant Pigment Chromatography and Photosynthesis
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Students perform thin layer chromatographic separation of carotene, xanthophyll and chlorophylls. Thin layer separation results are more defined than paper chromatography and minimize solvent use. Includes key reagents to demonstrate photosynthesis. Note: some perishable items require freezer storage. For 10 lab groups. Duration 90 minutes or two lab periods.
Kit contents:
Chloroplast extraction buffer
Reducing reagent
Chloroplast dilution buffer
DCIP
(2, 6-dichlorophenol-indophenol) concentrate
Standard pigments
Chromatography solvent
Sarkosyl ionic detergent
Thin layer chromatography plate
Capillary pipets
Transfer pipets
Microtest tubes
Centrifuge tubes
Instructions
Required, but not included: visual spectrophotometer, clinical centrifuge, flood light or small portable lamps, beakers, test tubes and racks, 1 and 5 mL pipets with pumps or bulbs, metric rulers, cheesecloth, foil, pencils, 95 to 100% isopropanol, distilled water, ice, fresh spinach, blender, calculator, timer, 95-100% isopropynol, distilled water, ice, and personal safety equipment.
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