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Lab 4: Plant Pigment Chromatography and Photosynthesis
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Students perform thin layer chromatographic separation of carotene, xanthophyll and chlorophylls. Thin layer separation results are more defined than paper chromatography and minimize solvent use. Includes key reagents to demonstrate photosynthesis. Note: some perishable items require freezer storage. For 10 lab groups. Duration 90 minutes or two lab periods.
Kit contents:
- Chloroplast extraction buffer
- Reducing reagent
- Chloroplast dilution buffer
- DCIP
- (2, 6-dichlorophenol-indophenol) concentrate
- Standard pigments
- Chromatography solvent
- Sarkosyl ionic detergent
- Thin layer chromatography plate
- Capillary pipets
- Transfer pipets
- Microtest tubes
- Centrifuge tubes
- Instructions
Required, but not included: visual spectrophotometer, clinical centrifuge, flood light or small portable lamps, beakers, test tubes and racks, 1 and 5 mL pipets with pumps or bulbs, metric rulers, cheesecloth, foil, pencils, 95 to 100% isopropanol, distilled water, ice, fresh spinach, blender, calculator, timer, 95-100% isopropynol, distilled water, ice, and personal safety equipment.
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