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AP® Biology Investigation #9: Restriction Enzyme Analysis of DNA


In this lab students will understand the action and specificity of restriction enzymes, while
learning the components involved in the process of DNA electrophoresis and the role of
each component as it applies to the entire process of electrophoresis. Students will perform
the electrophoresis process on DNA samples treated with different restriction enzymes and
construct a standard curve using a known DNA sample while determining the approximate
size of the DNA fragments in unknown samples. Meets AP Science Practices 3 and 6, and
Big Idea 3. Materials provided for eight lab groups.


   
 

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