Keep my session open?
Ending In 
The session is expired
Your session has expired. For your security, we have logged you out.
Would you like to log in again?

pBR322 from Escherichia coli, MP Biomedicals

pBR322 is a 4363 bp vector purified from Escherichia coli.

  • Liquid, soluble in water
  • Supplied in a 10 mM Tris-HCl, 1 mM EDTA buffer at a concentration of 0.5 ug/ul
  • pH 7.5
  • Store at –20 °C
It is one of the most commonly used cloning vectors, that confers resistance to ampicillin and tetracycline. It contains single restriction sites for Pst I and Pvu I within the ampicillin-resistance gene (ampr) and single restriction sites for BamH I, Sal I, Sph I, EcoR V, Nru I and Xma III within the tetracycline-resistance gene (tetr).

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication. Note-Elevated temperatures and/or multiple freeze/thaw cycles will cause some of the ccDNA to convert to ocDNA.

pBR322 DNA exists in supercoiled (closed circular) DNA (ccDNA) and linear DNA (both open circular pBR322 plasmid adn E. coli chromosomal contamination) (ocDNA) in an approximate ratio of 90:10.



Item # Description Size Unit Availability Price Quantity
470202-882 pBR322 from E. coli
25 µg Each Retrieving



Need help?

  • Whatever you have to say, positive or negative, is important to us.
  • Providing feedback takes only seconds.
  • Your feedback will be made available to the public on the web. will publish your name as user.
You can use this name, or edit and save a nickname.
We recommend authors use their first and last initial.

Please confirm your profession

Example: Education

Please confirm your role

Example: Teacher

Please confirm your location

Example: New York, NY


Total Ratings: 0
Avg. Ratings: 0.0 out of 5

Sort all by:

Review & Compare Alternatives
We found alternative products that can save you up to  per item-unit.  To compare product details, select up to 3 alternatives below and click Compare Selected. To add items to your basket, enter a quantity and click Add to Basket.

Original Product:

Description Catalog Number Availability Unit Your Price Price Per Qty