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Ward's® Agriculture and the Environment LC50: How Much is Too Much Nitrate? Lab Activity

AD-CC0266-7KIT WARD470180-306 WARD470184-484
Exciting Environmental Chemistry

  • Students Observe the Effects of a Chemical on an Organisms in Real Time
  • Cross-Discipline between biology, chemistry and environmental science
Become part of a scientific team assigned to conduct a bioassay demonstrating the effects of ammonium nitrate on Daphnia magna. Using a fluorescently-tagged sugar, you will examine the effects of ingestion of different concentrations of ammonium nitrate by the Daphnia. The investigation will allow students to determine which Daphnia are healthy and which are adversely affected by exposure to ammonium nitrate. After a healthy Daphnia eats the specially tagged sugar, the fluorometric marker is cleaved from the sugar during digestion and circulates throughout the organism's system. This animal will fluoresce under long wave ultraviolet light. The adversely affected Daphnia will be unable to ingest or digest the sugar, and as a result will emit little or no light. The goal of the bioassay is to determine the LC50 value of ammonium nitrate within the Daphnia. In order to ensure that you have the freshest materials to complete this activity, please be sure to order the following prior to performing this activity: 87-5218: Daphnia magna, Mixed Sizes and 250-8236: MUG Vial.

Copper Sulfate Solution
Medicine Cups
Disposable Pipettes
Food Coloring
Plastic vials with caps
Plastic cups

Required but not Included: UV Light (Black light), UV Protective Goggles, Beaker or small container, distilled water, and scissors.



Item # Description Includes No. of Lab Groups Unit Availability Price Quantity
470118-184 Agriculture and the Environment: LC50 How Much is Too Much Nitrate? Lab Activity
Hazardous Product
Teacher's Guide and Student Copymaster 12 Each Retrieving



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