Enables Both Quantitative and Qualitative Measure of Reactions
- Includes materials for eight student workstations with 2-4 students per group
- Aligns with AP Biology Big Idea 4
- Includes Teacher's Guide, Student Manual, and Graphic Quick Guide
Reveal the power of enzyme kinetics by illustrating their function through the real-world application of biofuel production. This kit allows students to measure the enzymatic activity of cellobiase (part of the cellulase family) and identify the optimal conditions for the enzyme. The reaction of cellobiase breaking down cellobiose is important in the process of making cellulosic ethanol, which is an efficient, more sustainable fuel to replace petroleum. Students test and calculate the conversion rate of a sugar substrate (p–nitrophenyl glucopyranoside) to p–nitrophenol and glucose in the presence or absence of cellobiase. One complete 45-minute core activity plus five additional enrichment activities enable instructors to tailor coursework across several disciplines at a variety of levels.
Activities:
Activity 1 (core): Determine the reaction rate in the presence or absence of an enzyme
Activity 2: Determine the effect of temperature on the reaction rate
Activity 3: Determine the effect of pH on the reaction rate
Activity 4: Determine the effect of enzyme concentration on the reaction rate
Activity 5: Determine the effect of substrate concentration on the reaction rate
Activity 6: Test the ability of mushroom extracts to increase the reaction rate
Kit Contents:
Enzyme, cellobiase, 400 μl
Substrate, p-nitrophenyl glucopyranoside, 90 mg
Standard, p-nitrophenol (1 mM, 4 ml)
2x stop solution, 100 ml
10x resuspension buffer, 50 ml
Extraction buffer, 50 ml
Disposable plastic transfer pipets
1.5 ml microcentrifuge tubes
15 ml conical tubes
Disposable cuvettes
Instruction manual
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