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Bio-Rad® Comparative Proteomics Kit I: Protein Profiler


Explore Evolution and Construct Cladograms

  • Lab can be completed in three 45 minute lab sessions
  • Includes materials for eight setups
This kit moves beyond DNA and allows your students to employ protein gel electrophoresis to explore evolution at the molecular level. Students generate protein profiles from the muscle tissues of distantly and closely related species of fish to test the hypothesis that protein fingerprints can indicate evolutionary relatedness. Changes in proteins can reflect changes in the gene pool. Detectable variations among organism's protein profiles reflect physiological adaptations to different environments, but variations originate as random DNA mutations. Such mutation events, if favorable, persist through the natural selection process and contribute to the evolution of species with new specialized functions. Based on their own results, students can decide whether their results support biological evolution. Precast TGX gels required, not included.

Kit Contents:
Laemmli sample buffer, 30 ml
Precision Plus Protein™ Kaleidoscope™ standards, 50 ml
10x Tris/Glycine/SDS electrophoresis buffer, 1 L
Bio-Safe™ Coomassie stain for proteins, 100 ml
Actin and myosin standard, 500 mg lyophilized
Dithiothreitol (DTT), 0.3 g
Prot/Elec™ pipet tips for gel loading
1.5 ml fliptop microcentrifuge tubes
1.5 ml screwcap microcentrifuge tubes
Disposable plastic transfer pipets
Gel staining trays
Foam floats
Curriculum including teacher's guide, student manual, and graphic quick guide

Required Accessories Not Included in Kit:
Fish samples 5-8 types
Adjustable micropipets, 2-20 ml
Power supplies
Water bath or dry bath

Required Accessories If Using Polyacrylamide Gel Electrophoresis:
Vertical gel electrophoresis chambers
Ready Gel® or Mini-PROTEAN® TGX™ precast polyacrylamide gels, 15%, 10-well each

Required Accessories If Using Agarose Gel Electrophoresis:
Horizontal gel electrophoresis chambers
Low-melt agarose, 25 g
Pipet tips, 2-20 ml
Acetic acid
Reagent alcohol/ethanol


   
 

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Item # Description No. of Lab Groups Unit Availability Price Quantity
470017-228 Comparative Proteomics Kit I: Protein Profiler
8 Kit of 1 Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 1-800-727-4368 or email sargentwelchcs@vwr.com.

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