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pGLO™ Bacterial Transformation Kit


Students can transform bacteria with the jellyfish gene.

  • Relevant, Real-World Activities
  • Kit Aligns with AP Biology Lab 6
  • Supplies and Techniques Used in Today's Most Advanced Biotech Labs
  • Estimated Lab Time: 90 Minutes Over Two Days
Renowned as a premier name in biotechnology education, Bio-Rad Laboratories offers research-level lab activities that are accessible to high school and college students. Each exemplary experiment is designed to bring trusted, reliable, and repeatable results to your lab. Now, with the combined expertise of Ward's Natural Science and Bio-Rad, you can provide students with authentic activities and apparatus that prepare them for future biotechnology study, applications, research, and careers.

Genetic engineering is the process of manipulating the genetic material of an organism. This process is often used to introduce DNA from a foreign organism into another. In the pGLO bacterial transformation kit, students transform bacteria with a gene from a bioluminescent jellyfish to express a new protein, green fluorescent protein (GFP). They can turn the genes on and off to study gene regulation. This same procedure is used for creating designer proteins and has led to the explosion of new medicines, agricultural applications, and environmental solutions. Aligned with AP Biology Lab 6, this lab can be completed in two 45-minute lab sessions.

Ordering Information: This kit includes plasmid (pGLO) (lyophilized), e. coli strain HB101 (lyophilized), sterile LB nutrient broth, LB nutrient agar powder, ampicillin (lyophilized), arabinose (lyophilized), sterile transformation solution, sterile petri dishes (60 mm), sterile inoculation loops, 2 mL sterile microcentrifuge tubes, color-coded foam floats, disposable plastic transfer pipets, and a UV pen light. A hot-water bath or hot plate, beakers, and ice are needed but not included.

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Item # Description No. of Lab Groups Unit Availability Price Quantity
470160-726 pGLO™ Bacterial Transformation Kit
8 Kit of 1 Retrieving Restricted
470018-258 Manual
Each Retrieving Restricted
WLS1775-79 Manual
Each Retrieving Restricted
restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 1-800-727-4368 or email sargentwelchcs@vwr.com.

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