Simulate Testing for the FH Gene
- Convenient Ready-To-Load samples
- Connection to heart disease
- Inquiry-based experiment
- Time required: ~45 minutes
Elevated blood cholesterol has been established as a serious risk factor for coronary heart disease and stroke which are leading causes of death in the United States. A disease known as familial hypercholesterolemia (FH) causes an increase in blood levels of the ""bad"" form of cholesterol, known as low density lipoprotein (LDL). In this experiment, a simulated genetic test for FH is demonstrated in which patients are tested for a DNA polymorphism linked to the FH gene.
Kit includes: instructions, Ready-to-Load™ QuickStrip™ DNA samples, UltraSpec-Agarose™ powder, practice gel loading solution, electrophoresis buffer, InstaStain® Blue and FlashBlue™ stain, calibrated pipette, and microtipped transfer pipettes.
Required, but not included: electrophoresis apparatus, power supply, automatic micropipette and tips, balance, microwave or hot plate, visualization system.