Students learn about DNA fingerprinting in a dry lab setting.
- Students Construct Specific Genes with the Characteristic Nucleotide Configuration for Cystic Fibrosis
- Kit Shows the Difference between Normal and Mutant Genes
- Students Learn How Disease Can Be Diagnosed By Two Methods Of DNA Analysis
- Time Required: One 50 Minute Class Period
- Teacher's Guide Included
This kit introduces students of biotechnology, forensic science, or AP Biology to the concepts of DNA replication and how it relates to PCR (Polymerase Chain Reaction) and RFLP (Restriction Fragment Length Polymorphism) analysis of DNA.
Students observe how DNA analysis can also be used to diagnose disease. This activity is a great introduction to or review of DNA fingerprinting.
Ordering information: This kit includes a teacher’s guide; student worksheets and guides; nucleotides sheet: cytosine 28 (blue); nucleotides sheet: guanine 28 (yellow); nucleotides sheet: adenine 66 (red); nucleotides sheet: thymine 66 (green); DNA stands, labels, sheets; mutant CFTR; normal CFTR; primer 1; primer 2; template-mutant CFTR; template-normal CFTR; electrophoresis bands, labels, sheets; mutant cftr pcr product (22 bp); mutant CFTR RFLP product (10 bp); mutant CFTR RFLP product (14 bp); normal CFTR PCR product (25 bp); normal CFTR RFLP product (25 bp); adhesive strips sized for 4 bp, 30 18 bp, 30 21 bp; adhesive strips sized for 22 bp, 3 25 bp; DNA polymerase; glue sticks, removable; bags, re-sealable; taql restriction enzyme; and work boards, two-sided.