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Purification and Size Determination of GFP/BFP Kit (EDVO-Kit 255)

Students use Green Fluorescent Protein (GFP) to identify the localization and function of proteins in cells.

  • Material for Six Groups Sharing Three Gels
  • Fluorescent Property of GFP Functions as Assay
  • GFP Tags Proteins in Cells
  • Optional Activity Included
  • Some Components Require -20°C Storage
GFP has been isolated and cloned from the jellyfish Aequorea victoria for this kit. The protein gives a green fluorescent glow under a long UV light, and it has been used as a tag to follow proteins in cells. In this experiment, the unique fluorescent property of GFP is used as an assay during its purification from an E. coli extract. Column fractions that contain GFP are identified by green fluorescence. As an optional activity, purified protein fractions can be submitted to denaturing SDS polyacrylamide gel analysis to estimate the purity and size of the GFP protein.

This kit includes material for six groups sharing three gels. Vertical gel electrophoresis apparatus, a power supply, 5—50 μL micropipets, and polyacrylamide gels are required but not included.

Delivery information: The kit is shipped at room temperature, but some components require -20°C storage upon receipt.



Item # Description Unit Availability Price Quantity
470112-846 Purification and Size Determination of GFP/BFP Kit
Each Retrieving Restricted

Temperature sensitive This Item is temperature-controlled and may have specific temperature and storage requirements for shipping/delivery.

restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 1-800-727-4368 or email


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