This kit provides a hands-on examination of immunology procedures.
- Students Practice Immunology Procedures
- Time Required: ~75 min over Two Days
- Instructions Included
The second assay used to confirm a positive HIV ELISA result is the Western Blot.
Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane, and detect the simulated HIV proteins.
Ordering information: This kit includes instructions, samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 mL pipette, and an 100 mL graduated cylinder. Electrophoresis apparatus, power supply, micropipettes with tips, a microwave or hot plate, an incubation oven, a shaker platform, lab glassware, small plastic trays, micro test tubes, pipette pumps or bulbs, metric rulers, distilled water, methanol, and glacial acetic acid are required but not included.