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Thin-Layer Chromatography Lab Activity

This kit illustrates the principles of Thin Layer Chromatography (TLC).

  • Kit Familiarizes Students with Thin Layer Chromatography
  • Students Learn How to Establish Rf Standards
  • Material for 24 Students
  • Estimated Lab Time: One Lab Period
The lab techniques involve silica-gel-coated plastic slides, indicators, developers, and chromatography chambers. Each student runs chromatograms of five different sample dye solutions to establish Rf (Rate of flow) standards. They also run chromatograms of two “unknowns,"" which are identified for the teacher in the instruction booklet. This approach gives students an opportunity to appreciate the usefulness of TLC. TLC, which is similar and complementary to paper chromatography, can also be employed to determine the purity of a system.

Ordering information: This kit includes TLC solvent, Developing chambers with caps, TLC layers, Capillary applicators, Indigo carmine, Methyl blue, Sudan IV, Methyl violet, Methyl orange, and Unknowns I and II.



Item # Description Includes No. of Lab Groups Unit Availability Price Quantity
470200-070 Thin-Layer Chromagtography Lab Activity
Teacher Guide, SDS, Student Guide, Worksheets 12 Kit of 1 Retrieving Restricted
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