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Western Blot Analysis Kit

317
Protein identification is based on both antibody reactions and antigens in this Western Blot activity.

  • Materials for Six Blots Included
  • Instructions with Background Information
  • Students Research Protein Identification
A denaturing SDS polyacrylamide gel separates the proteins, which are transferred or blotted to a nylon membrane. The membrane is then exposed sequentially to solutions containing the primary antibody, followed by a secondary antibody that is coupled to an enzyme.

The membrane is soaked in a substrate solution to develop the color reaction, which results in identifying the antigen as a band, and the molecular weights of the visible bands are measured using pre-stained protein markers.

Ordering information: This kit contains negative lyophilized control BSA, high and low concentration (lyophilized); an anti-BSA protein antibody; secondary antibody conjugate; hydrogen peroxide; peroxide co-substrate, 10X; blocking buffer; PBS buffer; pre-stained protein standard marker (lyophilized); tris-glycine-SDS electrophoresis buffer; powdered milk; and instructions with background information. The kit requires but does not include three polyacrylamide gels, MV-10 vertical gel electrophoresis apparatus, DC power supply, automatic micropipet, fine micropipet tips, glacial acetic acid, methanol, and distilled or deionized water.


Delivery information: This kit is shipped ambient. Store lyophilized proteins at room temperature.


   
 

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Item # Description Unit Availability Price Quantity
470120-900 Western Blot Analysis Kit
Each Retrieving Restricted

Temperature sensitive This Item is temperature sensitive and has specific temperature and storage requirements for shipping/delivery. Shipping duration cannot exceed 2 days.

restricted This product is marked as restricted and can only be purchased by approved Shipping Accounts. To apply for a shipping account number, click here. If you need further assistance, call Customer Service at 1-800-727-4368 or email sargentwelchcs@vwr.com.

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